I love food with healthy and simple ingredients. I am a recipe developer and food blogger who is inspired by many cultures around the world. Today, I could be in Thailand having a Tom Yum Soup, and tomorrow I will be in France eating bouillabaisse!
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The abnormal regulation of apoptosis can result in uncontrolled cell division, leading to diseases such as cancer. Thus, inducing apoptosis is regarded as one of major mechanisms for cancer therapy . The main feature of apoptosis is the activation of caspase cascades, which is essential for apoptotic cell death in both intrinsic and extrinsic pathways . Caspase-3, the most critical apoptosis downstream performer of caspase cascade, is known to catalyze the cleavage of PARP and actively induce cellular apoptosis . Our results revealed that E7050 induced apoptosis in MES-SA/Dx5 cells as evidenced by increasing the levels of cleaved caspase-3 and PARP. It has been reported that the tumor suppressor p53 plays a major role in the control of apoptosis . Several p53-inducible genes, which are involved in apoptosis and cell cycle arrest, have been identified, such as Bax and p21 [33,34]. Meanwhile, apoptosis-related proteins, also known as Bcl-2 family members, such as anti-apoptotic protein Bcl-2, and pro-apoptotic protein Bax, also regulate apoptosis . In this study, we found that treating MES-SA/Dx5 cells with E7050 resulted in an elevated level of p53 protein in MES-SA/Dx5 cells. Upon activation of the p53-dependent apoptosis pathway, the pro-apoptotic protein Bax was also upregulated. Moreover, the IAP protein survivin is highly expressed in most cancers, and has been shown to inhibit apoptosis, enhance proliferation and promote angiogenesis . Our study demonstrated that E7050 treatment could result in the downregulation of survivin, which contributes to the anti-cancer effect of E7050 through inhibition of cell proliferation and activation of apoptosis. d2c66b5586